Expression profiling identifies responder and non‐responder phenotypes to interferon‐β in multiple sclerosis

Expression pro®ling identi®es responder and non-responder phenotypes to interferon-b in multiple sclerosis

1Neuroimmunology Branch, National Institute of Neurological Disorders and Stroke, 2Metabolism Branch, National Cancer Institute, 3Laboratory of Diagnostic Radiology Research, Clinical Center,National Institutes of Health, Bethesda, MD, USA, 4SBU Therapeutics, Schering AG, Berlin, Germany Correspondence to: Roland Martin, MD, Neuroimmunology Branch, NINDS, National Institutes of Health, Bldg 10,...

Gene Expression Profiling of Multiple Sclerosis Pathology Identifies Early Patterns of Demyelination Surrounding Chronic Active Lesions

In multiple sclerosis (MS), activated microglia and infiltrating macrophages phagocytose myelin focally in (chronic) active lesions. These demyelinating sites expand in time, but at some point turn inactive into a sclerotic scar. To identify molecular mechanisms underlying lesion activity and halt, we analyzed genome-wide gene expression in rim and peri-lesional regions of chronic active and in...

Autoantibody Profiling in Multiple Sclerosis

Adaptive differentiation of murine lymphocytes. IV. (Responder × Nonresponder) F(1) T cells can be taught to preferentially help nonresponder,rather than responder, B cells

Responses to the synthetic terpolymer L-glutamic acid, L-lysine, L-tyrosine (GLT) in the mouse are controlled by H-2-1inked Ir-GLTgenes. (Responder x nonresponder) F(1) hybrid mice, themselves phenotypic responders, can be primed with GLT to develop specific helper cells capable of interacting with 2,4-dinitrophenyl hapten (DNP)-primed F(1) B cells in response to DNP-GLT. Unlike the indiscrimin...

Protein array-based profiling of CSF identifies RBPJ as an autoantigen in multiple sclerosis.

OBJECTIVE To profile the reactivity of CSF-derived immunoglobulin from patients with multiple sclerosis (MS) against a large panel of antigens, to identify disease-specific reactivities. METHODS CSF from subjects with MS with elevated immunoglobulin G and CSF from control subjects presenting with other inflammatory neurologic disease were screened against a protein array consisting of 9,393 p...